We all know that molecules are too small to be seen so we’re often in the dark about what a sample consists of, how pure it is or how quickly it’s reacting. With spectrophotometry we can use light to ‘see’ all of these aspects, spectrophotometry is therefore a widely used technique. Working in small teams we extracted chlorophyll from spinach, then used serial dilution to prepare a range of samples of known concentrations. Spectrophotometric analysis of these samples allowed us to construct a standard curve, we could then use this to work out the concentration of an unknown sample.
This workshop was good for
- Understanding how spectrophotometry works
- Practising extraction techniques and serial dilutions
- Establishing good spectrophotometer technique
- Constructing standard curves and understanding how to use them
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