Workshop 5: Connect the dots: Can your dilution make a perfect line?

We all know that molecules are too small to be seen so we’re often in the dark about what a sample consists of, how pure it is or how quickly it’s reacting. With spectrophotometry we can use light to ‘see’ all of these aspects, spectrophotometry is therefore a widely used technique. Working in small teams we extracted chlorophyll from spinach, then used serial dilution to prepare a range of samples of known concentrations. Spectrophotometric analysis of these samples allowed us to construct a standard curve, we could then use this to work out the concentration of an unknown sample.

This workshop was good for

  • Understanding how spectrophotometry works
  • Practising extraction techniques and serial dilutions
  • Establishing good spectrophotometer technique
  • Constructing standard curves and understanding how to use them

Find out more and register to attend a session today by visiting our registration page .

Want to know more about spectrophotometry before attending the workshop? Check out these videos on spectrophotometry and standard curves.

To contact the Kitchen Chemistry team email success.health@qut.edu.au .